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Measurements of the microphysical properties of mixed-phase clouds  with high spatial resolution are important to understand the  processes inside these clouds. This work describes the design and  characterization of the newly developed ground-based field  instrument HOLIMO II (HOLographic Imager for  Microscopic Objects II). HOLIMO II uses  digital in-line holography to in situ image cloud particles in  a well-defined sample volume. By an automated algorithm,  two-dimensional images of single cloud particles between 6 and  250 μm in diameter are obtained and the size spectrum,  the concentration and water content of clouds are calculated. By  testing the sizing algorithm with monosized beads a systematic  overestimation near the resolution limit was found, which has been  used to correct the measurements.    Field measurements from the high altitude research station  Jungfraujoch, Switzerland, are presented. The measured number size  distributions are in good agreement with parallel measurements by  a fog monitor (FM-100, DMT, Boulder USA). The field data shows that  HOLIMO II is capable of measuring the number size distribution with  a high spatial resolution and determines ice crystal shape, thus  providing a method of quantifying variations in microphysical  properties. A case study over a period of 8 h has been  analyzed, exploring the transition from a liquid to a mixed-phase  cloud, which is the longest observation of a cloud with  a holographic device.  During the measurement period, the cloud does  not completely glaciate, contradicting earlier assumptions of the  dominance of the Wegener–Bergeron–Findeisen (WBF) process

HOLIMO II: a digital holographic instrument for ground-based in situ observations of microphysical properties of mixed-phase clouds