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. Primary biological aerosol particles (PBAPs) in the atmosphere are highly relevantfor the Earth system, climate, and public health. The analysis of PBAPs,however, remains challenging due to their high diversity and largespatiotemporal variability. For real-time PBAP analysis, light-inducedfluorescence (LIF) instruments have been developed and widely used inlaboratory and ambient studies. The interpretation of fluorescence data fromthese instruments, however, is often limited by a lack of spectroscopicinformation. This study introduces an instrument – the Spectral IntensityBioaerosol Sensor (SIBS; Droplet Measurement Technologies (DMT), Longmont,CO, USA) – that resolves fluorescence spectra for single particles and thuspromises to expand the scope of fluorescent PBAP quantification andclassification. The SIBS shares key design components with the latest versions of theWideband Integrated Bioaerosol Sensor (WIBS) and the findings presented hereare also relevant for the widely deployed WIBS-4A and WIBS-NEO as well asother LIF instruments. The key features of the SIBS and the findings of thisstudy can be summarized as follows. Particle sizing yields reproducible linear responses for particles in therange of 300 nm to 20  µ m. The lower sizing limit is significantlysmaller than for earlier commercial LIF instruments (e.g., WIBS-4A and theUltraviolet Aerodynamic Particle Sizer; UV-APS), expanding the analyticalscope into the accumulation-mode size range. Fluorescence spectra are recorded for two excitation wavelengths ( λex=285 and 370 nm) and a wide range of emission wavelengths( λmean=302 –721 nm) with a resolution of 16detection channels, which is higher than for most other commerciallyavailable LIF bioaerosol sensors. Fluorescence spectra obtained for 16 reference compounds confirm that theSIBS provides sufficient spectral resolution to distinguish major modes ofmolecular fluorescence. For example, the SIBS resolves the spectraldifference between bacteriochlorophyll and chlorophyll a and b . A spectral correction of the instrument-specific detector response isessential to use the full fluorescence emission range. Asymmetry factor (AF) data were assessed and were found to provide onlylimited analytical information. In test measurements with ambient air, the SIBS worked reliably and yieldedcharacteristically different spectra for single particles in the coarse modewith an overall fluorescent particle fraction of ∼4  %( 3σ threshold), which is consistent with earlier studies incomparable environments.

Spectral Intensity Bioaerosol Sensor (SIBS): an instrument for spectrally resolved fluorescence detection of single particles in real time