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. Atmospheric simulation chambers are exploratory platforms used to studyvarious atmospheric processes at realistic but controlled conditions. Wedescribe here a new facility specifically designed for the research onatmospheric bio-aerosol as well as the protocols to produce, inject, exposeand collect bio-aerosols. ChAMBRe (Chamber for AerosolModelling and Bio-aerosol Research) isinstalled at the Physics Department of the University of Genoa, Italy, and itis a node of the EUROCHAMP-2020 consortium. The chamber is made of stainlesssteel with a total volume of about 2.2 m 3 . The lifetime of aerosolparticle with dimension from a few hundreds of nanometres to a few micronsvaries from about 2 to 10 h. Characteristic parts of the facility are theequipment and the procedures to grow, inject, and extract bacterial strainsin the chamber volume while preserving their viability. Bacteria are part ofthe atmospheric ecosystem and have impact on several levels as: healthrelated issues, cloud formation, and geochemistry. ChAMBRe will hostexperiments to study the bacterial viability vs. the air quality level, i.e.the atmospheric concentration of gaseous and aerosol pollutants. In thisarticle, we report the results of the characterization tests as well as ofthe first experiments performed on two bacterial strains belonging to theGram-positive and Gram-negative groups. A reproducibility at the 10 %level has been obtained in repeated injections and collection runs with aclean atmosphere, assessing this way the chamber sensitivity for systematicstudies on bacterial viability vs. environmentalconditions.

ChAMBRe: a new atmospheric simulation chamber for aerosol modelling and bio-aerosol research