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. We describe a systematic approach to the calibration and uncertaintyestimation of a high-resolution continuous flow analysis (CFA) water isotope(δ2H, δ18O) record from the Roosevelt IslandClimate Evolution (RICE) Antarctic ice core. Our method establishes robustuncertainty estimates for CFA δ2H and δ18Omeasurements, comparable to those reported for discrete sampleδ2H and δ18O analysis. Data were calibratedusing a time-weighted two-point linear calibration with two standardsmeasured both before and after continuously melting 3 or 4 m of ice core.The error at each data point was calculated as the quadrature sum of threefactors: Allan variance error, scatter over our averaging interval (error ofthe variance) and calibration error (error of the mean). Final mean totaluncertainty for the entire record is δ2H=0.74 ‰ and δ18O=0.21 ‰.Uncertainties vary through the data set and were exacerbated by a range offactors, which typically could not be isolated due to the requirements of themulti-instrument CFA campaign. These factors likely occurred in combinationand included ice quality, ice breaks, upstream equipment failure,contamination with drill fluid and leaks or valve degradation. We demonstratethat our methodology for documenting uncertainty was effective across periodsof uneven system performance and delivered a significant achievement in theprecision of high-resolution CFA water isotope measurements.

Calculating uncertainty for the RICE ice core continuous flow analysis water isotope record