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Adipocyte differentiation, which plays an important role in fat deposition, involves a complex molecular mechanism. MicroRNAs (miRNAs) are essential in this progress. Here, we showed that miR-25-3p expression had increased during goat intramuscular preadipocyte differentiation, which peaked at day 3. Using liposome transfection and qRT-PCR techniques, we found that knocking down miR-25-3p reduced the accumulation of lipid droplets by downregulating or upregulating the expression of LPL , PPAR   γ  , AP2 , SREBP1 , and C/EBP   β  but upregulating the expression of KLF4 . Overexpression of miR-25-3p results in the opposite. Furthermore, the dual luciferase assay showed that overexpression of miR-25-3p significantly inhibited luciferase activity of KLF4 . These results showed that miR-25-3p has a binding site within the 3'   -UTR of KLF4 mRNA. Together, these findings indicate that miR-25-3p is a positive regulator of intramuscular preadipocyte differentiation via targeting to KLF4 in goats.

MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting &lt;i&gt;KLF4&lt;/i&gt;

MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting <i>KLF4</i>