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Acyl-CoA synthetase long-chain family member 5 ( ACSL5 ) is a member of the acyl coenzyme A (CoA) long-chain synthase families (ACSLs), and it plays a key role in fatty acid metabolism. In this study, we proved an association between the  ACSL5  gene and triglyceride metabolism at the cellular level in cattle. pBI-CMV3- ACSL5  and pGPU6/GFP/Neo- ACSL5  plasmids were constructed and transfected into bovine preadipocytes by electroporation. The expression level of  ACSL5  was detected by real-time quantitative PCR and western blot. The triglyceride content was detected by a triglyceride kit. The results indicated that the expression level of  ACSL5  mRNA and protein in the pBI-CMV3- ACSL5 -transfected group was significantly increased compared with those in the control group. Furthermore, the pGPU6/GFP/Neo- ACSL5 -transfected group was significantly decreased compared with those in the control group. A cell triglyceride test showed that overexpression or silencing of the  ACSL5  gene could affect synthesis of cellular triglycerides. This study investigated the mechanism of ACSL on bovine fat deposition, and also provides a new candidate gene for meat quality traits in beef cattle.

The effect of acyl-CoA synthetase long-chain family member 5 on triglyceride synthesis in bovine preadipocytes