English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This study was carried out to screen the GDF9 gene and evaluate thepolymorphism effect on litter size of four Iranian sheep breeds usingthe PCR-RFLP and PCR-SSCP methods. First, sequencing of the GDF9 gene in16twin-birth, 4 triplet-birth, and 2 infertile ewes showed that, in addition to G2,G3, G4, G5, and G6 mutations that have been previously reported in other breeds,a new G0 mutation, called C25T, exists in the GDF9 sequence of 1 out of 22 ewes and causes L9F substitution in the signal peptide region. None ofthe triplet-birth or infertile ewes carried G1, G4, G7, FecGE, G8,or FecGT mutations. In the second experiment, a large dataset was used:605 individuals including 496 ewes (145 Afshari, 54 Shal, 126 Ghezel, and 171 Lori-Bakhtyari sheep), and 109 rams (26 Afshari,23 Shal, 10 Ghezel, and 50 Lori-Bakhtyari sheep. There were nosheep carrying the G7, G8, or Thoka mutations. Among all 109 rams that wereused in this study, none of them were homozygous for the G1 mutation.Moreover, abundance of heterozygote rams (G1/G+) varied from 0.0 (Afshari)to 28.6 % (Lori-Bakhtyari and Ghezel). The highest and the lowestfrequencies of the G4 mutation were 30.6 and 3.0 % in Shal and Afshari breeds,respectively. Moreover, G4 abundance varied from 0.0 to 42.3 %, from 3.0to 26.9, and from 3.0 to 30.6 % in rams, ewes, and overall, respectively.There was a significant difference in the abundance of G1 and G4 mutationsbetween breeds. However, neither the G1 nor the G4 mutation was associated with littersize in Afshari, Ghezel, Lori-Bakhtyari, or Shal sheep breeds. In conclusion, theresults of this study showed that GDF9 G1 and G4 mutations are not the reason forhigher litter size in Iranian sheep. Moreover, the GDF9 G0 and G6mutations do not cause triplet births or infertility in Iranian ewes.Therefore, it is unlikely that variant GDF9 mRNA induces larger litter size orinfertility in Iranian ewes

archives animal breeding/archiv für tierzucht

Variant &amp;lt;i&amp;gt;GDF9&amp;lt;/i&amp;gt; mRNA is likely not the main cause of larger litter size in Iranian Lori-Bakhtyari, Shal, Ghezel, and Afshari sheep breeds