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Cryopreservation of testicular tissueis a new option in fertility preservation for prepubertal male animals. Thepurpose of this study was to explore the effects of different cryoprotectantagents (CPAs) at various concentrations on testes after the cryopreservationof calf testicular tissue. These experiments selected dimethyl sulfoxide(DMSO), glycerol, propylene glycol (PrOH), and sucrose as CPAs in varyingdoses (2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 %; v/v) in 8-month-oldcalf testicular tissue that was frozen and preserved. Then, cell viability,testosterone production, malondialdehyde (MDA)level, and superoxide dismutase (SOD) level were detected and analyzedfollowing cryopreservation. The results showed that the optimalconcentrations of DMSO, PrOH, glycerol, and sucrose were 10, 10, 7.5, and10 %, respectively. Compared to the optimal concentrations ofCPAs, cell viability andtestosterone production decreased significantly at a lowerand higher CPAconcentration (P < 0.05). At the optimal concentrations of CPAs,the DMSO group showed higher cell viability and testosterone production thanother CPA groups (P < 0.05). Compared to the optimal concentrationof CPAs, the MDA level increased and the SOD level decreased at a lower orhigher concentration of CPAs, but there was no significant difference (P> 0.05). Cell viability was significantly positively correlatedwith testosterone production (P < 0.05). In conclusion, DMSOprovided the most effective protection for calf testicular tissuecryopreservation and the optimal concentration was 10 %

Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue