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The aim of the present study was to determine the effects of breed (NakedNeck – NN – and Lohmann Brown – LB), age (46 to 49 weeks) and the time of day of sampling(morning eggs – M – and afternoon eggs – A) on the proximate and fatty acidcomposition of whole eggs. Eggs of commercial hens (LB) had significantly (P < 0.001) less fat,ash and protein and lower dry-matter content (more water content) comparedto the autochthonous breed (NN). The NN eggs contained significantly (P < 0.001)more SFA (saturated fatty acid) and less PUFA (polyunsaturated fatty acid) than LB ones, while the share of theeggs' total n-3 fatty acids did not differ significantly between breeds. The shareof total MUFA (monounsaturated fatty acid) significantly (P = 0.011) decreased, while the share of totalPUFA, n-6 and n-6 / n-3 ratio significantly increased (P < 0.001;P < 0.001; P = 0.032, respectively) with age of hens. Additionally,compared with morning eggs, afternoon eggs had a significantly (P = 0.046)higher share of total n-6 fatty acids. PCA (principal component analysis) offered a good separation of the samples according to breed andage when two first principal component were extracted. PC1 was positivelyrelated to parameters of proximate composition and SFA content, while PC2was positively determined by PUFA, n-6 and n-3 content. Eggs ofautochthonous hens (NN) were located in the positive area of PC1,whereas those from the commercial hens (LB) were in the negative quarter,which indicates opposite characteristics

Effects of age and time of day of sampling on proximate and fatty acid composition of whole eggs from two strains of laying hens