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The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk inextenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawedartificial insemination (AI) doses, and during thermodynamic testing compared to extendersnot containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each ofthe 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMedand Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A totalof 640 AI doses were analysed. The volume of samples, sperm concentration, and percentageof motile spermatozoa were evaluated after collection, as was sperm motility after thawingof AI doses and during thermodynamic testing. Percentages of live and dead sperm were alsoevaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). Theresults confirmed significant (P<0.05–0.01) between-sire differences in the volume, density,and activity of sperm as well as in share of live and dead sperm after collection; in decline ofsperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamictest, as well as in the share of live and dead sperm after thawing. The extenders ranked bysperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higherquality of AI doses produced using egg yolk extenders. Differences in sperm motility weresignificant (P<0.05–0.01) during the entirety of thermodynamic testing. Egg yolk extendershad a significantly (P<0.05–0.01) higher percentages of live sperm after thawing

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing