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In this study the protein and mRNA expression of Monocarboxylate transporter 1 (MCT1) wasevaluated in rumen epithelial cells (REC) obtained from sheep fed hay <i>ad libitum</i> (control, hdiet, n=4) or a mixed hay/concentrate diet (h/c diet, n=4) for two weeks. REC were isolatedvia fractionated trypsination and three groups consisting of fractions 3 to 5=G1, fractions 6 to8=G2, and fractions 9 and 10=G3 were formed. Using an anti-basal cytokeratin antibody andflow cytometric analysis, the proportion of REC originating from the <i>stratum basale</i> (SB) wasdetermined for each group. In addition, MCT1 mRNA and protein expression was determinedby qRT-PCR and Western blot, respectively. Feeding the h/c diet led to a 299±93 % elevationof the number of SB cells known to express the MCT1 protein. This is accompanied by anincreased MCT1 mRNA (1.8 to 2.2-fold) and protein (1.3-fold) expression. Thus, an increasednumber of MCT1 expressing cells and upregulation of ruminal MCT1 protein seem to becomponents of rumen epithelium functional adaptation to high energy diet

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The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay &amp;lt;i&amp;gt;ad libitum&amp;lt;/i&amp;gt; diet (Short Communication)