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The influence of milk withdrawal, stable routines and separation from dam on suckling behaviour of beef calveswas tested in an 8x8 Latin square experiment. Length of the meal and length of the longest bout were measuredto describe the meal as a whole. Length of pre-stimulation, increasing ejection, declining ejection, and afterstimulationwere measured to describe the structure of meal. Eagerness of suckling was described as relativesuckling time and non-suckling. Milk withdrawal increased length of meal and longest bout, but did notinfluence structure of meal. Milk withdrawal resulted in cistern-milk being available already before ejection andthereby in longer bouts during pre-stimulation. Milk withdrawal had no influence on eagerness of suckling.Stable routines had no influence on meal as a whole, but increased pre- and decreased after-stimulation andtended to result in somewhat longer bouts during pre-stimulation. There was no influence of stable routines oneagerness of suckling. Separation from dam had no influence on meal as a whole or structure of meal, butincreased eagerness of suckling for the whole meal and for almost all periods. The experimental results partiallysustained results from a field study

The influence of milk withdrawal, stable routines and separation from dam on suckling behaviour of Hereford calves