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Bali cattle are one of Indonesian native cattle’s that famous for their ability to adapt to tropical harsh conditions. For the last 25 years, indiscriminate crossbreeding using artificial insemination (AI) organizing mainly by government might be has contaminated the purity of Bali cattle. In order to utilize as well as to conserve Bali cattle it is necessary to develop an accurate and simples method to detect the purity of Bali cattle. This experiment is the continuation of a long term experiments in developing methods to detect the purity of Bali cattle i.e. phenotypic variations, blood protein polymorphisms, hair structure, chromosome and DNA microsatellite variation analyses. The specificity of HEL9 and INRA035 microsetellites in Bali cattle as well as in Banteng (<i>Bos sondaicus</i>) as their ancestor was tested.  The results show that A and B alleles at INRA035 microsatellite locus are monomorphic and can be used for a specific markers for Bali cattle. Allele A at locus HEL9 that has high frequency (92.90%) in Bali cattle and 100% in Banteng can also be used as a supporting marker

The Use of HEL9 and INRA035 Microsatellites as Specific Markers for Bali Cattle