English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The reduction of milk yield caused by heat stress in summer is the main condition restricting the economic benefits of dairy farms. To examine the impact of hyperthermia on bovine mammary epithelial (MAC-T) cells, we incubated the MAC-T cells at thermal-neutral (37°C, CON group) and hyperthermic (42°C, HS group) temperatures for 6 h. Subsequently, the cell viability and apoptotic rate of MAC-T cells, apoptosis-related genes expression, casein and amino acid transporter genes, and the expression of the apoptosis-related proteins were examined. Compared with the CON group, hyperthermia significantly decreased the cell viability ( p &lt; 0.05) and elevated the apoptotic rate ( p &lt; 0.05) of MAC-T cells. Moreover, the expression of heat shock protein ( HSP ) 70 , HSP90B1 , Bcl-2-associated X protein ( BAX ), Caspase-9 , and Caspase-3 genes was upregulated ( p &lt; 0.05). The expression of HSP70 and BAX (pro-apoptotic) proteins was upregulated ( p &lt; 0.05) while that of B-cell lymphoma (BCL)2 (antiapoptotic) protein was downregulated ( p &lt; 0.05) by hyperthermia. Decreased mRNA expression of mechanistic target of rapamycin (mTOR) signaling pathway-related genes, amino acid transporter genes ( SLC7A5 , SLC38A3 , SLC38A2 , and SLC38A9 ), and casein genes ( CSNS1 , CSN2 , and CSN3 ) was found in the heat stress (HS) group ( p &lt; 0.05) in contrast with the CON group. These findings illustrated that hyperthermia promoted cell apoptosis and reduced the transport of amino acids into cells, which inhibited the milk proteins synthesis in MAC-T cells.

Effect of hyperthermia on cell viability, amino acid transfer, and milk protein synthesis in bovine mammary epithelial cells