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Amitrole is a widely used polar pesticide, with many difficulties to be analyzed at the regulatory level in environmental water, because its high solubility in water and low volatility. In this study, we propose a novel direct detection method of amitrole based on the chemiluminescent reaction with tris (2, 2'-bipyridine) ruthenium (III) after reversed-phase ion-pair HPLC separation. The detection limit of amitrole was of 0.25μg/l (signal-to-noise ratio=3) without preconcentration and derivatization steps. The method was successfully applied to the real sample of river water spiked with 1μg/l amitrole.

Determination of Amitrole with Chemiluminescent Reaction Using Electrogenerated Tris(2,2'-bipyridine)ruthenium (III)