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Due to decreases in dehydroepiandrosterone (DHEA) levels in an age group where there is increased incidence of chronic degenerative diseases, like diabetes mellitus type 2, we sought to identify the effect of incubation with DHEA on insulin secretory and cytoprotective capacity in pancreatic beta cells. The INS-1E cells were incubated in different concentrations of DHEA (10 µM, 100 nM and 1 nM) or vehicle (dimethyl sulfoxide; DMSO) for 1 or 7 days. Insulin secretion stimulated by glucose (2.8 mM and 16.7 mM), determination of superoxide content, and the protein content of insulin receptor substrate-2 (IRS-2), extracellular regulated kinase 1 and 2 (ERK ½), phosphorylated extracellular kinase 1 and 2 (pERK ½), phosphorylated c-Jun Nterminal kinase (pJNK), cleaved caspase-3 and pancreatic duodenal homebox-1 (PDX-1) were analyzed. We observed no effects of DHEA incubation on insulin secretion. In contrast, DHEA incubation improved total insulin content. Moreover, we have observed no influence on the production of superoxide. In addition, an increased PDX-1 and reduced cleaved caspase-3 concentration were observed. Our data point out to a stimulatory action of DHEA on insulin production and cytoprotective effects.

research in endocrinology

Dehydroepiandrosterone Increases Pancreatic Duodenal Homebox-1 (PDX-1) and Reduces Cleaved Caspase-3 Protein Expression in Insulin-Secreting INS-1E Cells