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The consumption of food containing biogenic amines is reported to be responsible for many pharmacological effects (diarrhea, headache, asthma, hypotension, etc.) which lead to several types of foodborne disease, including histamine poisoning, mainly from ingestion of certain species of fish (scombroid poisoning), and tyramine toxicity (cheese reaction). Nevertheless, the occurrence of symptoms that result from such interactions remains hypothetical, because there are no acceptable clinical studies that demonstrate such reactions. One reason is the lack of reliable test methods. In the project Bioamina we contribute to this issue developing a multiplex diagnostic tool that is capable of reliably and simultaneously measuring histamine, tyramine, putrescine and other biogenic amines in test food, serum and urine. Herein, we present first results on the isolation and characterization of new diamine oxidases and a proof-of-concept for biogenic amine detection via H2O2 production.

P2AR.11 - Colorimetric Multiplex Assay for Rapid Detection of Biogenic Amines