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Cortisol is one of the key hormones that is involved in stress response and an indicator of chronic psychological disorders. It is produced by zona fasciculata and can be found in blood serum and saliva. This study describes the fabrication of cortisol biosensor using molecular imprinted polymers (MIPs) as recognition unit. The 642.3±35.8 nm-core shell cortisol-MIP was prepared by methacrylic acid and acrylamide with cortisol as a target molecule in a ratio of 2:1:1. Electron and atomic force microscope techniques were used to characterize the physical properties of cortisol-MIP. The binding characteristics were evaluated using covalent patterns changing in Fourier transform infrared (FTIR) spectra and massive response by quartz crystal microbalance (QCM) technique. Cortisol-MIP was integrated into the sensor and coated on the disposable screen-printed carbon electrode (SPCE) for portable electrochemical analysis, which displayed 1.28-20mM detection range and high selectivity compared to three other steroid analogs.

BS5.1 - Biosensor of stress hormone cortisol based on molecular imprinted polymer