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This contribution describes the development and characterization of a multi-analyte biosensor system for simultaneous detection of up to four different analytes. The bi-enzymatic detection principle enables facile determination of ethanol, formate, Dand L-lactate. For immobilization of the required enzymes, a chemical cross-linking step with glutaraldehyde was applied. Combination of various analyte-specific electrodes, within one biosensor array, provides a broader insight into the complex nature of fermentation processes. Application of the biosensor chip for detection of organic acids in real samples, such as silage (maize and sugar cane) and fermentation sludge from a biogas plant, is presented. Comparison with a conventional reference technique demonstrates a promising potential of the multi-analyte biosensor for rapid monitoring of complex media.

BS3.5 - Multi-analyte Biosensor Chip for Determination of Organic Acids in Biogas Processes