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Amperometric flow-injection analysis (FIA)-type glucose biosensor was fabricated using porous carbonfelt modified with glucose oxidase (GOx) and horseradish peroxidase (HRP). Enzyme-immobilization (adsorption) protocol and operational parameters were optimized. The obtained GOx/HRP-CF-based FIA-type glucose biosensor showed the sensitivity (2.07 μA/mM), the limit of detection (0.014 mM), and linear range (0.03 to 3 mM). Interference by ascorbic acid (0.5 mM) could be cancelled by the control of applied potential. The RSD for 63 consecutive injection of 10 mM glucose was 3.71 %. The biosensor was applied for the determination of glucose in various beverages, and the analytical results obtained by the sensor were in fairly good agreement with those by conventional enzyme-based spectrophotometry.

BS3.3 - Enzyme-modified carbon-felt based flow-type amperometric glucose biosensor using oxidase/peroxidase-bienzyme system