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Phospholipase D (PLD) is an important enzyme in regulating biological processes. A polydiacetylene (PDA) with imidazolium as head group was developed as the sensor of PLD activity in this work. PLD catalyzed the hydrolysis reaction of L-phosphatidylcholine to produce phosphatidic acid, the negative charges of phosphatidic acid interacted with positive charges of imidazolium, which caused the color transition from blue to red and turn-on fluorescence. No obvious changes of PDAs were observed in absence of PLD or in the presence of PLD coexisting inhibitor. Furthermore, paper based PDAs sensor was constructed and exhibited convenient and sensitive detection for PLD activity.

BS3.2 - Polydiacetylene-based sensors for the activity assay of phospholipase D