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The determination of L-ascorbic acid (AA) by a high throughput chemiluminescence (CL) method using a metal ion attached multiwalled carbon nanotubes (MWCNTs) immobilized microfluidic chip has been developed. The method is based on enhancing CL intensity of the luminol-hydrogen peroxide system with the immobilized metal-MWCNTs in the microfluidic chip and on the quenching of CL intensity with addition of AA. The effects of pH, concentrations of luminol, hydrogen peroxide and metal ions on the CL intensity were investigated and optimized. The calibration curve for AA was linear over the range of 1.6×10 -8 M to 6.4×10 -7 M, the correction coefficient was 0.98913 and the detection limit was 1.11×10 -9 M.

P1.4.13 Determination of L-Ascorbic Acid by a Chemiluminescence Method using a Metal-MWCNT Immobilized Microfluidic Chip