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This study reports the development of two enzyme-less biosensors by using IrOx for pH and Pt-Ir for glucose sensors. Nanostructures of electrodeposited IrOx and Pt-Ir were examined by SEM and XPS on the electrode surfaces. We used chronopotentiometry (CP) for pH and linear sweep voltammetry (LSV) for glucose measurement at 37 C in 1X PBS (0.15M NaCl). In the physiological range of pH 4-10 and glucose concentration 1 mM-12 mM, these two biosensors exhibited good linearity of -68.5 mV/pH (R: 0.99) and, 0.97 mA/mM-dm (R: 0.988), respectively. Because the linear ranges are suitable for cell culture, the presented electrochemical sensors are prospective for applying to continuous cell culture monitoring.

7.2.6 IrOx and Pt-Ir Electrochemical Sensors: Prospective Enzyme-less pH and Glucose Sensors for Continuous Monitoring in Cell Culture