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An electrochemical in vitro sensor system is presented that reflects directly the metabolic activity of prokaryotic and eukaryotic cells. It can be used during a cultivation process to detect growth parameter, as a quality measurement system in food industry and also for the determination of toxic effects of chemicals on bacteria, vertebrate cells, cyanobacteria and algae. The evaluation is based on current-time curves of a potentiostatic measurement produced by the detection of the microbially reduced mediator molecule immobilised in a gel structure. Depending on the number of reduced mediator molecules a current signal is produced, which provides fast information about the viability of living cells. Different senor setups were developed depending on the target application. The final results of the electrochemical sensor show a good correlation with results from reference methods and also with values found in literature.

tagungsband

P2.11 - Activity of bacteria, mammalian cells, yeasts, cyanobacteria and alga determined by an electrochemical sensor: overview of different applications