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In this study, a scanning light-addressable potentiometric sensor (LAPS) was applied for label-free and direct electrical detection of DNA (deoxyribonucleic acid) hybridization for the first time. The functioning principle of the sensor is based on an electrostatic detection of the intrinsic molecular charge of DNA molecules together with an ion-concentration redistribution within intermolecular spaces. The LAPS signal shows a distinct dependence on the concentration of the complementary target DNA. The mechanism of the shift of the photocurrent-voltage curve and the LAPS signal generation induced by the DNA immobilization and hybridization event is discussed

A9 - An application of a scanning light-addressable potentiometric sensor for label-free DNA detection