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Microorganisms can easily degrade agro-industrial wastes in a solid-state fermentation process thereby synthesizing many important commercial bio compounds including lipolytic enzymes. Screening different habitats for bacteria with lipase activity and optimizing its fermentation parameters with facilities effective production and usage in industries. In this study, soil samples from different Abattoirs in Kaduna metropolis were screened for presence of lipase positive strains culture medium parameters such as carbon source, inducers, nitrogen source, pH, temperature, inoculums level and incubation period were varied for the purpose of optimization. Maximum lipase activity was recorded at 48hours incubation, this activity occurred in media containing groundnut peel and rice husk, supplemented with ammonium nitrate and urea which are the best carbon and nitrogen sources. The isolated bacteria were identified as Pseudomonas biochemically, the production, partial purification and characterization of lipase activity at different pH, determination of molecular weight analysis. The lipase was partially purified up to saturation using ammonium sulphate precipitation spectrophotometric lipase assay was used for enzyme characterization. Lipases produced by pseudomonas have been used widely in pharmaceutical, food, pulp, textile, paper, leather industries among others due to their stability, selectivity and broad substrate specificity.

Isolation, Optimization and Characterization of Lipase Producing Bacteria from Abbatoir Soil