English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Objective: Altough there are many microogranisms that might be the agents of gastroenteritis, routine laboratory tests can not determine all of them with our current techniques. This situation leads to failure of empirical treatment and to economical losses due to incorrect usage of antibiotics. In this study, we aim to investigate prevalence and antimicrobial susceptibility of Campylobacter and Salmonella from patients with acute gastroenteritis. Methods: Between 1 June 2015 and 31 May 2016, stool samples were inoculated on Campylosel agar (bioMérieux, Marcy l'Etoile, France) and GN enrichment broth (Merck, Darmstad, Germany). Campylosel agar incubated at 42°C for 48-72 hours in microaerophilic atmosphere. After 6-8 hours incubation at 37°C, GN enrichment broth was subcultured to EMB agar (Merck, Darmstad, Germany) and Hektoen enteric agar (Merck, Darmstad, Germany). In addition to conventional tests, matrixassisted laser desorption/ionization time-of-flight mass spectrometry confirmed the identification of Campylobacteror Salmonella-suspected colonies. Antibiotic suscebtibility testing of Campylobacter species was carried out with gradient test (Etest®, bioMérieux, Marcy l'Etoile, France), and results were evaluated according to EUCAST criteria. Results: Pathogenic microorganisms were isolated in 67 (17.7%) of 379 stool cultures. 42 (62.7%) of them were identified as Campylobacter and 25 (37.3%) of them were identified as Salmonella. Of 42 Campylobacter strains, 41 were identified as C. jejuni, and one was identified as C. coli. 40 (95.2%) of Campylobacter strains were susceptible to erythromycin, 28 (66.7%) were susceptible to tetracycline, and 16 (38.1%) were susceptible to ciprofloxacin. Conclusions: Campylobacter species were the most commonly Özet Amaç: Gastroenterit etkeni pek çok mikroorganizma bulunmasına rağmen rutin laboratuvar testleri günümüz teknikleriyle bunların hepsini belirleyememektedir. Bu durum ampirik tedavide başarısızlıklara ve yanlış antibiyotik kullanımından dolayı ekonomik kayıplara neden olmaktadır. Bu çalışmada akut gastroenterit tanısı almış hastalarda Campylobacter ve Salmonella sıklığının ve antimikrobik duyarlılıklarının araştırılması amaçlanmıştır. Yöntemler: 1 Haziran 2015-31 Mayıs 2016 tarihleri arasında gönderilen dışkı örnekleri Campylosel agar (bioMérieux, Marcy l'Etoile, Fransa) ve GN enrichment broth (Merck, Darmstad, Almanya)’a ekildi. Campylosel agar mikroaerofilik koşullarda 42°C’de 48-72 saat inkübe edildi. GN enrichment broth, 6-8 saat 37°C’de bekletildikten sonra EMB agar (Merck, Darmstad, Almanya) ve Hektoen enteric agar (Merck, Darmstad, Almanya)’a yayıldı. Campylobacter ya da Salmonella kuşkulu kolonilerin tür tanımları yapılmak üzere konvansiyonel yöntemlerin yanı sıra “matrix-assisted laser desorption/ionization time-of-flight mass spectrometry” uygulandı. Campylobacter türleri için antibiyotik duyarlılık testi gradyan testiyle (Etest®, bioMérieux, Marcy l'Etoile, Fransa) yapıldı ve sonuçlar EUCAST kriterlerine göre değerlendirildi. Bulgular: Toplam 379 dışkı örneğinin 67 (%17.7)’sinde patojen mikroorganizma izole edildi. Bunların 42 (%62.7)’si Campylobacter ve 25 (%37.3)’i Salmonella olarak tanımlandı. 42 Campylobacter suşundan 41’i C. jejuni, biri C. coli olarak adlandırıldı. Campylobacter suşlarının 40 (%95.2)’ı eritromisine duyarlılık gösterirken, 28 (%66.7) Campylobacter suşu tetrasikline ve 16 (%38.1)’sı siprofloksasine duyarlı olarak bulundu. Sonuçlar: Çalışmamızın sonuçları Campylobacter türlerinin Özgün Araştırma / Original Article

Prevalence and Antimicrobial Susceptibility of Campylobacter and Salmonella Species in Patients With Acute Gastroenteritis