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Objective: The objective of this study is to describe the potatodextrose agar (PDA) with rose-Bengal and chloramphenicol asa new and simple medium (R-PDA chloramphenicol agar) tofacilitate the detection of black yeast, Exophiala dermatitidis compared to ready-made conventional medium, namely roseBengal chloramphenicol agar. Methods: We prepared a new medium by adding chloramphenicol and rose-Bengal to readymade PDA. Results: This medium proves better growth of the black yeast in terms of increased colony size compared to commercial rose-Bengal chloramphenicol agar. The increase in colony size aids for distinguishing the slow growing black yeast from all the other filamentous fungi. Conclusion: Compared to traditional rose-Bengal chloramphenicol agar, R-PDA chloramphenicol agar is superior to isolate Exophiala dermatitidis among other fast growing filamentous fungi which are present in the environment samples.

“Clean Care for All – It's in Your Hands”: 5th May 2019 World Health Organization SAVE LIVES: Clean Your Hands Campaign