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Large amount of passion fruit seeds are misspent after juice production. Heat and light can cause loss or reduction of seed oil antioxidant and antitumor activities. Thus, co-precipitation with polymers by Supercritical AntiSolvent process (SAS) may be used to preserve important compounds. This work aimed to study the phase behavior of the systems 1) CO2+ dichloromethane+ seed oil and 2) CO2+ dichloromethane+ seed oil+ PLGA (poly(lactic-co-glycolic) acid), to determine the operational conditions to be employed on future SAS assays. The static synthetic method was applied using different CO2 compositions (60 98.8 wt %) and temperatures (35 and 45 °C). Liquid-vapor, liquid-liquid and liquid-liquid-vapor phase transitions were observed. Conditions suggested to proceed the SAS encapsulation study are: 35 °C to preserve extract quality, CO2 concentration between 92.5 and 97.0 %, ensuring its action as anti-solvent, and pressure above 75 bar to guarantee a single phase equilibrium.

PHASE BEHAVIOR OF (CO2 + DICHLOROMETHANE +PASSION FRUIT SEED OIL) AND (CO2 +DICHLOROMETHANE + PASSION FRUIT SEED OIL + PLGA)SYSTEMS