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This study was conducted in the College of Agricultural Engineering the University of Baghdad, the apiary of the protection department and laboratory of the Department of Food Sciences on Apis mellifera hybrid honeybee colonies during the period from October 2019 to February 2020. The main study aim was isolating the bacteria genus Lactobacillus from the stomachs of honeybees and identifying them by performing morphological and microscopic examinations as well as conducting physiological and biochemical tests. The results of morphological examinations indicated that the colony is oval-shaped, shiny, with a milky white color, and its diameter does not exceed 1 mm. The microscopic examination results showed that the bacteria were gram-positive, not motile, and not forming spores. Whereas the physiological examinations proved that, the bacteria are anaerobic, inability to grow at temperatures of 5 and 15 oC, while they grow at a temperature of 45 oC and grow in saline media by 4% and 5%, while they do not grow in a saline media 6%. Finally, the biochemical test results showed that the bacteria are catalase-negative, do not consume citrate, do not reduce nitrates, do not produce ammonia from arginine, and have the ability to ferment certain sugars.

ISOLATION AND IDENTIFICATION OF BACTERIA GENUS LACTOBACILLUS AND COUNTING THEIR NUMBERS FROM THE HONEY BEE APIS MELLIFERA STOMACHS