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An experiment was conducted during the spring 2019 in one of experimental fields of Agriculture/University of AlQadisiyah, to study the response of two common beans Phaseolus vulgaris L varieties (Pole and Bush) to the biofertilization with Rizobium phaseoli sp with or without the presence of two mineral elements (iron and molybdenum) and their interactions, and the treatment effect on formation of root nodules and their chemical content. The experiment factors included two beans varieties (Pole and Bush), bio-fertilizer of Rhizobium phaseoli (inoculated and non-inoculated), nanoscale elements (iron 80mg.L, molybdenum 10 mg.Lmolybdenum) and all the possible interactions. The seeds were treated with bio-fertilizer before planting, while the Nano-scale elements treatments were applied one month of after planting. Measurements were taken at the end of the growing season, at harvest stage. The results showed that the use of bio-fertilizers and nanoscale elements improved the chemical content and the traits of the root nodules. The results showed that the plants treated with bio-fertilizer (inoculated) resulted in higher values and significantly differed from the untreated with bio-fertilizer (non-inoculated) by recording the highest averages for all the studied traits including number of root, nitrogenase enzyme activity, Amino acid tryptophan average, nitrates concentration ,iron concentration and molybdenum concentration . The addition Nano-elements also led to significant increase in the studied traits especially the combined treatment (iron 80 +molybdenum 10) mg.L as the treatment with the mixture recorded the highest rates. In case of plant varieties, the variety Bush performed higher than the Pole in all the studied traits except for the molybdenum concentration in the nodule and the amount of nitrates in the nodule. Best results of were obtained from interaction of Nano-elements mixture and bio-fertilizer with Bush variety followed by the same interaction with pole.

EFFECT OF BIO-FERTILIZER AND NANOSCALE ELEMENTS ON ROOT NODULES FORMATION AND THEIR CHEMICAL CONTENT OF TWO PHASEOLUS VULGARIS L VARIETIES