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Salmonella is a significant foodborne pathogen worldwide. This study was studied in vivo to estimate the lethal dose and infective dose of Salmonella typhimurium isolated from a child suffering from diarrhea, using mice white (BALB/c) of both sexes with an age range from six to eight weeks old, which administrated orally. The mice were observed daily for thirty days, the rested forty-eight mice divided randomly into eight groups each with six mice. The seven groups of mice administrated orally with one of the calculated (CFU/ml) diluents by using needle gavage about (0.5ml) and the eight groups administrated phosphate buffer saline (PH=7.2) and deemed as a control group. The result evidenced the LD50 was (1x10 8 cells) and infective dose (ID50) was (1x10 6 cells).

ESTIMATION OF LD50 AND ID50 DOSES OF SALMONELLA TYPHIMURIUM INOCULATED EXPERIMENTALLY IN MICE