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The present study aimed to produce silver nanoparticles by the Biosynthesis method which was a very important area due to their economic and ecofriendly benefits. The current research was used the whey of local isolates of Lactobacillus spp. were isolated and identified from different types of local dairy products in Baquba city markets. Silver nanoparticles were Characterized using X-ray diffraction (XRD) analysis confirms the nanoparticles are silver and silver chloride (AgCl) cubic types and by using the(crystalline) domains equation, three strongest peaks, the calculated particle size were 17.1and 18.6 nm for Ag, and 24.3 nm for and AgCl. Atomic Force Microscopy (AFM) is used for size, topography, and granularity volume distribution of biosynthesized nanoparticles Ag/Agcl average size 50 nm. UV-visible spectroscopy revealed the formation of AgNPs. Fourier transform infrared (FTIR) spectroscopy shows different functional groups of biomoleculesare responsible for the reduction and capping process. Electron microscopic (SEM) was used to characterize the shape and size of the nanoparticles. Determination of the antibacterial activity of silver and silver chloride nanoparticles against the selected multiple drugs resist (MDR) bacteria by agar well diffusion method. It was observed that the growth of these bacteria was inhibited from 12.5 mg/ml Ag & Agcl NPs concentration and the nanoparticles concentrations (12.5,25,50,100 mg/ml) had a maximum inhibitory effect against S. aureus (24, 28, 30, 32 mm respectively) and minimum inhibitory effect against E. coli (16, 21, 23, 27 mm respectively) while the others bacteria (P. mirabilis, S. epidermidis, K. pneumoniae, and P. aeruginosa) inhibitory effect varied between the bacterial isolates and the concentration of the silver nanoparticles.

PRODUCTION AND CHARACTERIZATION OF SILVER NANOPARTICLES BY LACTOBACILLUS SPP. AND DETERMINE INHIBITORY EFFECT AGAINST GROWTH OF PATHOGENIC BACTERIA