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Objectives: This study evaluated the angiogenic efficacy of gene delivery by intramuscular (IM) injections of VEGF 165 plasmid in an animal model. Material and methods: 36 rats underwent intramuscular injections of plasmid to hind limbs. The control group (18 animals) received empty plasmid. Group 1 received plasmid containing genes for vas cular endothelial growth factor (VEGF). After 12 weeks, all the animals were sacrificed and tissue sam ples from the thigh muscle from hind limbs were harvested for histological and immunohistochemistry examinations. Results: There were significant differences in the histological findings in the structure of muscle tissue of the hind limbs between two groups. There was evidence of angiogenesis in the samples from group 1. There was no angiogenesis present in the control group. The expression of VEGF 165 protein in the immunohistochemistry examination demonstrated gene delivery to muscles of hind limbs in group 1. Levels of expression of VEGF protein in the control group were not increased. The histological struc ture of the samples from group 1 confirmed successful gene delivery to muscles of hind limbs. Conclusions: Intramuscular injections of plasmid of VEGF induced angiogenesis and increased the concentration of VEGF protein in muscle tissue.

Angiogenesis after plasmid VEGF165 gene transfer in an animal model