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The study deals with the examination of the metabolic activity of neutrophils tested with spontaneous and stimulated (fMLP, PMA, Zymosan) chemiluminescence (CL) in children with type 1 diabetes depending on their clinical conditions. We examined 87 children with type 1 diabetes from 4 to 21 years (52 girls and 35 boys, mean age – 13 years). Due to their clinical conditions, the patients were divided into three subgroups: patients in good condition (25 children), patients in mediocre condition (54 children), and patients in severe condition (8 children). The control group consisted of 23 persons – healthy volunteers from 20 to 25 years old (mean age – 23 years). We estimated the maximum levels of chemiluminescence (CLmax) with a luminometer (Microlumat LB 96P of E&G Barthold), the results were given in RLU/s. We found decreased oxide metabolism of neutrophils in patients with diabetes in all clinical conditions, both spontaneous and stimulated compared to the control group. The lowest values of neutrophil chemiluminescence were in children in mediocre condition.

Clinical immunology Whole blood neutrophil chemiluminescence in children with diabetes depending on their clinical condition