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The sequencing of a protein is a complicated issue. Several methods have been developed in order to establish the amino acid sequences of proteins, including Edman degradation, LC/MS/MS and cDNA sequencing. A number of examples confirm that X-ray crystallography could also be a useful tool for the identification of an amino acid sequence of unknown proteins. Here, we present a short review summarizing the application of crystallography for protein sequence determination and identification.

Identification of amino acid sequences via X-ray crystallography: a mini review of case studies