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The suitability of in vitro culture technique for vegetative propagation of Biscutella laevigata calamine ecotype was investigated. Various organic additives were tested as medium supplements: squash obtained from fleshy pineapple fructification, liquid endosperm of coconut, and a conditioned medium acquired from the culture of green algae. Exploited plant-derived biostimulators along with growth regulators included in the propagation medium, improved B. laevigata multiplication in vitro. The highest micropropagation coefficient, exceeding three adventitious rosettes from one maternal microcutting, was obtained in the medium containing 10 ml A l !1 pineapple pulp during a 6-week-long culture. Microrosettes were rooted in vitro in a hormone-free, modified propagation medium, with macro- and microelements accounting for one third of the initial concentration. Acclimatization to ex vitro environment was conducted both in growth chamber and greenhouse conditions. Plants cultivated in the greenhouse bloomed and set seed. The results of the experiment allowed determination of the most suitable conditions required for culture initiation and successful development of a protocol for in vitro propagation of Biscutella laevigata ecotype obtained from the Olkusz district.

RESEARCH PAPER In vitro multiplication and acclimatization of Biscutella laevigata (Brassicaceae) to cultivation in greenhouse conditions