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This paper is dedicated to the memory of Professor Edward Pojnar (1919-2011), Head of the Department of Botany (1974-1990) at the University of Agriculture in Krakow, who initiated research on isolated plant protoplasts in Poland. Studying in Professor E.C. Cocking’s Plant Physiology Laboratory at the University of Nottingham, Professor Pojnar became acquainted with the enzymatic method procedure for protoplast isolation. His postdoctoral thesis was published in 1969 as the first paper on protoplast isolation and culture procedures in Poland. The present paper is a survey of Professor Pojnar’s research, as well as further studies inspired by his pioneering achievements. Recent research into protoplast technology, focused on the structural changes involved in protoplast regeneration, is also discussed.

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)