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The aim of this study is to explore serum norepinephrine (NE)concentration and β3-adrenoceptor (β3AR) protein expression at differenttimes during myocardial ischemia/reperfusion (I/R) in rats and to examinethe role of β3AR in I/R.    Material and methods  A  total of 28 Sprague-Dawley (SD) rats were randomly divided into one sham group and six I/R groups (6 h, 12 h, 18 h, 24 h,72 h, and 120 h). The rats in the I/R groups were subjected to ischemia for45 min. After reperfusion, the serum NE concentration and the β3AR proteinexpression in the myocardial tissue of the left ventricular injury region weredetected. Another 18 SD rats were randomly divided into a sham group, I/Rgroups, and an I/R + BRL37344 group. The myocardial infarct size (MIS) andthe expression of apoptotic and anti-apoptotic proteins in the left ventricular myocardium of each group were measured.    Results  Compared with the sham group, the serum NE concentration of ratsin the I/R groups was significantly higher at 6 h (p &lt; 0.001); the expressionof myocardial β3AR protein started at 12 h. The serum NE concentration andmyocardial β3AR protein expression were both highest at 72 h but remainedhigh in the 120-hour group. Compared with the sham group, the expressionlevels of the pro-apoptotic proteins Bax and cleaved caspase-3 after I/R weresignificantly higher (p &lt; 0.01, p &lt; 0.001, respectively), and the expressionof anti-apoptotic protein Bcl-2 was significantly lower (p &lt; 0.01). Comparedwith I/R groups, the expression levels of Bax and cleaved caspase-3 in theI/R + BRL37344 group were significantly lower (p &lt; 0.05, p &lt; 0.01, respectively), and that of Bcl-2 was up-regulated (p &lt; 0.05); MIS was significantlydecreased (p &lt; 0.001).    Conclusions  With the prolongation of myocardial I/R in rats, serum NE concentration and β3AR protein expression showed a  significant increasingtrend and reached a peak at 72 h. Specific β3AR agonist BRL37344 can reduce myocardial I/R injury in vivo in rats, alleviate apoptosis, reduce infarctsize, and improve cardiac function.

The expression and role of β3AR protein in myocardial ischemia/reperfusion in rats