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The aim of this study was to discuss the effects and mechanisms of lncRNA in renal injury induced by diabetes by vivo and vitro study. Material and methods Collecting healthy and DN patients, and measuring lncRNA ANRIL expression by RT-qPCR assay in serum. Measuring the ANRIL expression in normal, 10d and 20d model rats in kidney. Measuring LDH, MDA, SOD, IL-6 and TNF-α concentration by Elisa assay, evaluating the kidney pathology by HE staining, cell apoptosis by TUNEL assay, relative proteins expression by IHC assay and relative mRNA expression by RT-qPCR assay. In the cell experiment, Measuring LDH, MDA, SOD, IL-6 and TNF-α concentration by Elisa assay, measuring the cell proliferation by CCK-8, cell apoptosis by flow cytometry, relative mRNA and proteins expression by RT-qPCR and WB assay. The NF-κB(p65) nuclear volume of difference groups were evaluated by cell immunofluorescence. Results Compared with healthy people, lncRNA ANRIL was significantly increased in DN patients. Compared with sham group, the ANRIL expression, LDH, MDA, IL-6 and TNF-α concentration was significantly increased, SOD concentrations and cell apoptosis rate were significantly depressed with day increasing. TLR4, MyD88 and NF-κB(p65) mRNA and proteins expressions were significantly upregulation. Conclusions lncRNA ANRIL knockdown had effects to improve renal injury induced by diabetes via regulation TLR4/NF-κB(p65). Powered by TCPDF (www.tcpdf.org) Pr e rin t Long-coding RNA ANRIL knockdown improve diabetes-induced renal injury Xiaoming Jiang , Nan Su , Jiao Ding, Juean Jiang, Liqiang Yu, Yan Xie* 1. General Medicine Department, The First Affiliated Hospital of Soochow University 2. Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Soochow University *Corresponding Author, E-mail: xieyan200120@163.com #Both these authors contributed equally to this study Running title: ANRIL and kidney injury

lncRNA ANRIL play an important role in renal injury induced by diabetes