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This study aimed to investigate the preventive and therapeutic effects of crocetin (Cro) on ischaemic stroke in cell and animal models.    Material and methods  A  cell model of oxygen and glucose deprivation(OGD) and a rat model of middle cerebral artery occlusion were establishedto simulate ischaemic stroke. The infarct volume was measured by TTC assay, and the apoptotic cell number was counted by TUNEL. Relative proteinand gene expression levels in the rats were measured by immunohistochemical and RT-qPCR assays. The apoptosis rate and relative protein and geneexpression levels were determined by flow cytometry, WB and RT-qPCR assay, respectively.    Results  Compared with those in the normal control (NC) group, the braintissue injury and apoptotic cell number significantly increased (p &lt; 0.001)and the miR-145-5p gene expression significantly decreased in the cell andanimal experiments. In the animal experiment, the infarct volume, apoptoticcell number and pathological status improved in the Cro-treated groups.In the cell experiment, the apoptosis rates significantly depressed in theCro-treated groups (p &lt; 0.05). However, the cell apoptosis rate significantlyincreased after miR-145-5p inhibitor transfection (p &lt; 0.001). The proteinand gene expression levels of Toll-like receptor 4, myeloid differentiationfactor 88 and nuclear factor (NF)-κB (p65) significantly decreased (p &lt; 0.05).In addition, p-NF-κB(p65) nuclear volume significantly decreased (p &lt; 0.05).    Conclusions  Crocetin improved ischaemic stroke by regulating the miR145-5p/TLR4 axis in cell and animal experiments.

Crocetin improves ischaemic stroke in vitro and vivo