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Solid phase extraction coupled with spectrophotometric detection was applied to trace amounts of Pantoprazole (PP) drug using Cetyltrimethylammonium bromide coated-iron oxide magnetite nanoparticles CTAB@Fe3O4 MNPs. After characterization of the prepared nano-adsorbents, experimental parameters affecting the extraction efficiency of the developed method were optimized. The results obtained showed that this proposed approach is applicable in concentrations ranging from 0.1 to 1.5 μg/ml (R = 0.9958) indicating that follows Beer’s-Lambert law. The limit of detection and the limit of quantification calculated to be 0.014 and 0.04 μg/ml, respectively. The repeatability of the proposed method was evaluated with studying intra-day and inter-day precisions described by relative standard deviations. Eventually, two samples of natural waters as well as samples of human plasma were analyzed using the proposed method. Satisfactory precision and recoveries in the complex matrices were achieved.

nano biomedicine and engineering

Preconcentration and Determination of Pantoprazole by Solid-phase Extraction Coupled with Spectrophotometry Using Iron Oxide Nanoparticles Modified with Cetyltrimethylammonium Bromide