English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A novel bio-analytical method was developed and validated for the quantitative determination of raltegravir in rat serum by using the liquid-liquid extraction chromatography and tandem mass spectrometric detection (HPLC-MS/MS). Separation of raltegravir from the endogenous substances is achieved after liquid-liquid extraction by using HPLC-MS/MS system. Raltegravir was eluted in isocratic mode with acetonitrile, methanol and 0.1% acetic acid in water ( 40:30:30) at a flow-rate of 0.5 mL/min on Waters, Exterra C18, 50*4.6 mm, 5μm particle size column. Didanosine was used as the internal standard. The liquid-liquid extraction recovery was found 70% indicates good recovery. The validation results demonstrated that the present method was found to be precise and accurate. The stability tests indicated that the raltegravir in rat serum is stable for three freeze-thaw cycles at both -20 oC and -70 oC, 18-h ambient storage, 15-day frozen storage at both -20 oC and -70 oC. The results also showed no significant matrix effect (<6.2%). The present method was found to be sensitive and selective at very low levels of linearity range 11000 ng/mL, based on a sample volume of 50 μL, with a linear correlation coefficient of ≥ 0.99. The validated method has been successfully applied to support a preclinical pharmacokinetic study.

american journal of biomedical sciences

Development and Validation of a lc-ms/ms Method for the Determination of Raltegravir in Sprague Dawley Rat Serum and Its Application to Pharmacokinetic Study