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In this work, the multilayers film of myoglobin (Mb) and glutaraldehyde (GA) was assembled on the surface of gold electrode modified with MWCNTs by layer by layer (LBL) assembly technique, and the assembly process was characterized by cyclic voltammetry (CV) and UV-Vis spectroscopy. UV-Vis spectroscopy showed that Mb in the multilayers film retained its native biology activity, and the multilayers film exhibited good electrocatalytic activity toward reduction of H2O2 in pH 7.0 phosphate buffer solution (PBS), the currents intensity were linearly with concentrations of H2O2 in the range of 6×10 -6 to 8.4×10 -5 M with the detection limit of 5×10 -7 M (S/N=3). The Michaelis-Menten constant was 0.115 mM. In addition, interference experiments showed no interference response obtained for dopamine, ascorbic acid, ethanol, uric acid species et al at this working potential.

Fabrication of Multilayers Film of Myoglobin and Glutaraldehyde on the Surface of Gold Electrode Based on Covalent Interaction and Its Application