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Identification of sugar components and sugar linkages is required for structural determination of oligosaccharide chains. Several magnetization transfer methods, such as multiple relayed COSY and homonuclear Hartmann-Hahn methods are useful to extract subspectra of constituent monosaccharides. Individual monosaccharides give characteristic cross peak patterns due to their chemical shifts and spin-spin couplings so that we can identify the sugar types. The linkages of each sugar residue can be subsequently determined by the analysis of NOESY spectra. Thus, the chemical structure of oligosaccharide chains can be elucidated by non-empirical manner. We have applied this method to determine the chemical structure of oligosaccharide chains of a novel glycolipid from a sea urchin.

Structural Analysis of Oligosaccharide Chains of Glycolipids by Magnetization Transfer Methods.