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The growth patterns of Myc. lepraemurium that followed inoculation of crude and purified bacilli into subcutaneous tissues of mice were observed by means of the spread tissue preparations which was described by Kawaguchi. The growth patterns of the bacilli in mice were divided into two types; (1) the type of inoculation of crude bacilli; i.e. the growth of bacilli at the extracellular and intracellular sites. (2) the type of inoculation of pruified bacilli; i.e. only intracellular growth of bacilli. The former type includes the cases of inoculations of purified bacilli into dd-mice, inoculations of crude bacilli into C3H-mice, and inoculation of bacilli purified from rat leproma, containing subcutaneous tissues of C3H-mice and rat. On the other hand, experimental results obtained in the cases of inoculation of crude bacilli into dd-mice, and of bacilli purified from rat leproma, containing the subcutaneous tissues of rabbits and guinea-pigs, were belonged to the latter.

Growth Patterns of Crude and Purified Myc. Lepraemurium in Subcutaneous Tissues of Mice