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In the biochemical study of murine leprosy, the amount of fatty substances of various organs was examined and compared with the control, to investigate the disturbance of metabolism of infected rats. Animals employed in this experiment were five male white rats of 300-350 g, and five female of 200-250 g weight, after 4 months of murine leprosy bacillus inoculation and of similar weight and ten intact white rats of both sexes of similar weight were settled as control. The quantity of free and total cholesterol, cerebroside, Lecithin, Sphingomyelin, Cephalin, free fatty acids, nertral fats in N. ischiadicus, neck nodes, muscles of upper leg, subcutaneous fatty tissue, murine leproma, liver, lung, kidney and spleen was measured and compared with the normal. The results were shown in Table 2. Total cholesterol increases in the peripheral tissues and does not change in the visceral organs. Cerebroside increases in every tissue. Total phosphatide, especially Sphingomyelin, increases in the peripheral tissues and does not change in the viscera. Neural fat d ecreases in every tissue, while the changes of fatty substances are more remarkable in the peripheral tissues than in the internal organs. The above results indicate that murine leprosy is a general disease accompanied with the disturbances of metabolisms in various organs, of which detailed facts should be investigated.

STUDIES ON THE DEVIATION IN THE LIPID CONTENTS OF VARIOUS ORGANS AND TISSUES OF LEPROSY RATS