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Department of Bioinformatics, Korea Bio Polytechnic, Chungnam 320-905, Korea(Received March 22, 2011; Revised March 25, 2011; Accepted March 31, 2011).     (irradiation)            (zwitterion)   .              ,  Kelvin prove force microscopy (KFM)  .         ,                      .             XPS     .              ,                .    ,  ,             . : ,  , ,   ,  ABSTRACT. Merocyanine derivatives transformed from spiropyran-containing compounds by irradiating the light of ultra-violet (UV) include zwitterion of phenolate anion and amine cation. Complexation of this phenolate anion on merocyanine-modified surface and Ni ion among metal ions led to a change of surface charge and it was measured with kelvin prove forcemicroscopy (KFM). We found that the resultant surface potential decreased linearly as UV-exposed time increased, and finallywere saturated. Also it was analyzed through XPS the immobilized amount of Ni ions was increased according to increaseof UV-exposed time. It is considered that these properties could be applied for detection and a quantitative control of differentmetal ions. Further research is to aim construct specific scaffold/matrix which enable high selective, high sensitive and, espe-cially, a quantitative immobilization of metal ions-binding biomaterials such as proteins and cells.Keywords: spiropyran, photochromism, merocyanine, kelvin prove force microscopy (KFM), surface potential

Surface Modification Using Spiropyran-Derivative and Its Analysis of Surface Potential Induced by UV