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E-mail: yuzhifang@njau.edu.cnReceived June 9, 2014, Accepted July 5, 2014A new androstane derivative, 4β-methyl-15-oxa-14β-androstane-7-ene-4α-carboxylic acid (1) and a knownone 4β-methyl-15-oxa-14β-androstane-7-ene-4α-hydroxyl (2) were isolated from the EtOAc extract of thecultures of the fungus Marasmiellus ramealis (Bull.) Singer. Their structures were elucidated on the basis of1D and 2D NMR as well as MS spectroscopic data analysis. The inhibitory activity of two isolates againstacetylcholinesterase (AChE) revealed that compound 1 exhibited definitely inhibitory activity.Key Words : Marasmiellus ramealis (Bull.) Singer, Fermentation, Androstane derivative, AChE inhibitoryactivityIntroductionMarasmiellus ramealis (Bull.) Singer, belonging to thefamily Marasmiaceae, is a small, thin, white mushroom,usually growing on the deadwood. The fungi is an ediblefungus with a wide distribution in most parts of China,especially in Hainan, Hunan,Yunnan, and Tibet province.

Two Androstane Derivatives from the Cultures of Fungus Marasmiellus ramealis (Bull.) Singer