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E-mail: swcha@hufs.ac.kr Received April 8, 2013, Accepted April 23, 2013Matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a very effective method forlipid mass fingerprinting. However, MALDI MS suffered from spectral complexities, differential ionizationefficiencies, and poor reproducibility when analyzing complex lipid mixtures without prior separation steps.Here, we aimed to find optimal MALDI sample preparation methods which enable selective or class-wide massfingerprinting of two totally different lipid classes. In order to achieve this, various matrices with additives weretested against the mixture of phosphatidylcholine (PC) and cerebrosides (Cers) which are abundant in animalbrain tissues and also of great interests in disease biology. Our results showed that, from complex lipidmixtures, 2,4,6-trihydroxyacetophenone (THAP) with NaNO

Selective or Class-wide Mass Fingerprinting of Phosphatidylcholines and Cerebrosides from Lipid Mixtures by MALDI Mass Spectrometry